2X Super Hi-Fi Taq PCR MasterMix with loading dye
2× Super Hi-Fi Tag PCR MasterMix is a 2× concentrated, optimized mixture composed of Super Hi-Fi Tag DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer. The advantages of 2×Super Hi-Fi Tag PCR MasterMix include high convenience, sensitivity, specificity and stability. It minimizes man-made errors during PCR operating process. 2×Super Hi-Fi Tag PCR MasterMix is suitable for routine PCR reaction, amplification of complex templates such as GC rich templates (>60%) and templates with secondary structure.
Features
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Convenient : ready-to-use mix.
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A Blend of Taq DNA Polymerase with proofreading activity.
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Four-times greater accuracy than Taq DNA polymerase.
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Generates 3'-dA tailed PCR products.
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High yields of PCR products.
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Amplification of long targets up to 20 kb from genomic DNA.
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PCR products can be loaded directly without extra loading buffer.
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HSIEH, Yen‐Che, et al. Pparα deficiency inhibits the proliferation of neuronal and glial precursors in the zebrafish central nervous system. Developmental Dynamics, 2018, 247.12: 1264-1275.
Q1. Do you need to add additional dye for mastermix with loading dye?
A: No. but you still need to add DNA view or EtBr.
Q2. Does loading buffer effects downstream experiments?
A: No.
A: No. but you still need to add DNA view or EtBr.
Q2. Does loading buffer effects downstream experiments?
A: No.